The HARPIA-TF is a time-resolved fluorescence measurement module that combines fluorescence upconversion and TCSPC techniques. In fluorescence upconversion, the signal from the sample is mixed in a nonlinear crystal with a gating femtosecond pulse to achieve high temporal resolution, which is limited by the duration of the gate and pump pulses. For fluorescence decay times in the nanosecond to microsecond range, the instrument can be used in time-correlated single‑photon counting (TCSPC) mode to measure kinetic traces up to 5 μs. The combination of the two methods enables the measurement of spectrally-resolved fluorescence decay in the femtosecond to microsecond range. Using a high repetition rate PHAROS or CARBIDE laser, the fluorescence dynamics can be measured while exciting the samples with pulse energies down to several nanojoules.

HARPIA-TF – Kerr gate / Fluorescence Upconversion, and TCSPC

Extension Module	HARPIA-TF
Mode	Kerr gate	Fluorescence upconversion	TCSPC
Spectral range	250 – 1000 nm	330 – 1600 nm	320 – 820 nm ¹⁾
Temporal resolution	400 – 500 fs	≤ laser pulse duration or better	< 180 ps ²⁾
Max measurement range	8 ns		∞ ³⁾
Delay resolution	8.3 fs		–
Gate beam requirements	15 – 25 μJ		n/a
Compatible with	TCSPC		Kerr gate or fluorescence upconversion

Spectral range is extendable to NIR; contact sales@lighton.com for details.
High-speed detector available (< 50 ps); contact sales@lighton.com for details.
Maximum measurement range depends on the phosphorescence signal.

Dimensions

Extension Module	Physical dimensions (L × W × H)
HARPIA-TF	571 × 275 × 183 mm

Drawings

Drawing of HARPIA system with HARPIA-TB and HARPIA-TF modules

HARPIA optical layout for fluorescence upconversion measurements

Principle of time-resolved fluorescence upconversion

Principle of time-correlated single-photon counting (TCSPC)

HARPIA-TF – Kerr gate / Femtosecond Fluorescence Upconversion, and TCSPC Module

Fluorescence Upconversion Measurement Data Samples

Fluorescence dynamics of DCM laser dye in solution acquired using HARPIA-TF in fluorescence upconversion mode. Measurement conditions: 100 kHz repetition rate, 430 nm pump wavelength.

Kerr Gate Measurement Data Samples

Kerr gate measurements in DCM illustrate the method’s ability to probe fluorescence evolution with a sub-picosecond temporal resolution. Here we see how the fluorescence red-shifts during the early times of the spectral evolution.

Kerr gate measurements in beta-carotene generry showcase the resolution of the measurement. The S2→S0 fluorescence of carotenoids is ultrafast (<100 fs), therefore the measured data is more or less IRF-limited.

TCSPC Measurement Data Samples

Fluorescence dynamics of DCM laser dye in solution acquired using HARPIA-TF in TCPSC mode. Measurement conditions: 100 kHz repetition rate, 430 nm pump wavelength.

Publications

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Datasheets

Catalogs

Femtosecond Laser Systems for Science

Product catalog.

Rev. 16/09/2022. Size 15.2 MB.

飞秒激光器飞秒科研系统

Product catalog in Chinese.

Rev. 16/09/2022. Size 15.8 MB.

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HARPIA-TF Femtosecond Fluorescence Upconversion and TCSPC Module

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