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24 May, 2024
24 May, 2024

Three-Photon Microscopy Comes of Age

How does three-photon microscopy stack up against two-photon microscopy?

Image quality in 2P vs 3P microscopy as a function of depth in mouse brain.
Image quality in 2P vs 3P microscopy as a function of depth in mouse brain. Courtesy of Albrecht Stroh, University Medical
Center Mainz and Leibniz Institute for Resilience Research.

The use of high-energy, low-repetition rate excitation for deep imaging remained unexploited until 2013 when it was realized that three-photon microscopy  (3PM) leverages short, energetic pulses to a larger extent than two-photon microscopy (2PM). Since then, 3PM gained popularity as a tool able to extend the capabilities of 2PM by imaging deeper layers in the brain and other tissues such as tumors and bone.

Explore the potential of 3P imaging with Light Conversion’s microscopy dedicated CRONUS-3P femtosecond laser in the article published by Laser Focus World.

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